Skip to main content
. 2019 Nov 19;116(49):24630–24638. doi: 10.1073/pnas.1913992116

Fig. 2.

Fig. 2.

DNA encapsidation does not affect DNA accumulation. (A) The levels of viral DNA synthesized in 293 cells harboring WT EBV or 1 of 2 EBV mutants were measured by qPCR at 48 hpi. The mutants do not express either the portal protein, BBRF1, or the major capsid protein, BcLF1, and therefore cannot encapsidate viral DNA. The lytic phase was induced by transfection of plasmids encoding the immediate early genes BRLF1 and BZLF1. In addition, a plasmid encoding the late gene product p18 fused to GFP was transfected to evaluate the fraction of cells supporting the lytic phase (n [biological replicates] = 3, error bars indicate SD). An unpaired Student’s t test was carried out to determine P values (P [BcLF1 vs. WT] = 0.1; P [BBRF1 vs. WT] = 0.4). (B and C) The intracellular and extracellular encapsidated DNAs were assayed by measuring the levels of DNaseI-resistant P3HR1 and 4012 DNAs using qPCR at the indicated times from iD98HR1-4012 cells. n (biological replicates) = 3 (the shaded areas indicate the SD of the mean of the 3 biological replicates).