Table 1.
Impaired bacterial clearance of NOD1−/−, NOD2−/− MEs. No CFUs were detected by d 5 after NTHi inoculation in WT C57BL/6 mice. Bacterial clearance was impaired in NOD1−/−, NOD2−/− mice until 10 d after inoculation. NTHi was isolated from more than half of the MEs by d 7 and all bacteria cleared by d 14. Mean bacterial colonization of the culture positive plates was evaluated using a semi-quantitative analysis of bacterial colonization: 0 indicates no CFUs, 1 indicates one quadrant with CFUs, 2 indicates two quadrants with CFUs, 3 indicates three quadrants with CFUs, and 4 indicates four quadrants with CFUs. Data represent culture positive plates out of six.
| Time after NTHi inoculation | C57 WT mean bacterial CS | C57 WT CFU/ml | NOD1−/− mean bacterial CS | NOD1−/− CFU/ml | NOD2−/− mean bacterial CS | NOD2−/− CFU/ml |
|---|---|---|---|---|---|---|
| d 0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| d 1 | 3.00 | >104 | 2.5 | −103 | 2.5 | −103 |
| d 2 | 4.00 | >105 | 1 | −500 | 3.25 | >104 |
| d 3 | 1.00 | −500 | 2.5 | −103 | 2.5 | −103 |
| d 7 | 0.00 | 0.00 | 0.5 | −500 | 0.5 | −500 |
| d 10 | 0.00 | 0.00 | 0.1 | −150 | 0.2 | −300 |
| d 14 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |