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. 2019 Oct 9;244(16):1438–1445. doi: 10.1177/1535370219880490

Figure 2.

Figure 2.

Effect of T to C mutation for rs1982809 on BTLA mRNA stability and the activity of 3ʹ-UTR. (a) Transcriptional level of BTLA in peripheral blood of 10 rs1982809 CC-carrier patients and 10 rs1982809 TT-carrier patients was detected by RT-PCR. The peripheral blood volume was used corresponding to the same transcriptional level of BTLA to investigate the mRNA stability. Peripheral blood was treated with actinomycin D. Samples were removed at 2, 4, and 6 h. The values correspond to the mean ± SD. There were no differences in the BTLA mRNA levels between the CC-carrier patients and the TT-carrier patients (P = 0.86). (b) The luciferase activity of the transfected THP-1 was measured using a luciferase assay system. The relative luciferase activity (RLA) was significantly lower in the cells transfected with the construct containing the C allele than the cells transfected with the construct containing the T allele (**P < 0.01).