Table 1.
RT-PCR primers designed for triplex detection of porcine enteric viruses.
| Primer | Sequence (5′–3′) | Location | GenBank accession |
|---|---|---|---|
| PEDV-F | CTGCCAATGTATTTGCCAC | 21341-21359 | AF353511 (CV777) |
| PEDV-R | GGAAGTTCCTTGAACCTCG | 21999-21981 | AF353511 (CV777) |
| PSV-F | TGCTTGAGGAGTCGGAGAG | 5102-5120 | JX286666 (YC2011) |
| PSV-R | GCCCTGCACAACTGCTTTC | 5529-5510 | JX286666 (YC2011) |
| SaV-F | TACGGGGGAATAGGTTT | 5855-5871 | AF182760 |
| SaV-R | CAGCCACATCTGGGTAGT | 6100-6083 | AF182760 |
PEDV = porcine epidemic diarrhea virus; PSV = porcine sapelovirus; RT-PCR = reverse-transcription PCR; SaV = sapovirus.