Table 3.

Results of prospective and retrospective validation of the multiplex real-time PCR completed directly on clinical samples and compared to NVSL serotyping results.

n	Identification
	Culture result	rtPCR result
Prospective validation
16	No Salmonella	No Salmonella
4	S. 4,[5],12:i:-	S. 4,[5],12:i:-
4	S. Heidelberg	Salmonella, non-Typhimurium, non-4,[5],12:i:-
Retrospective validation
16	S. 4,[5],12:i:-	S. 4,[5],12:i:-
13	Salmonella, non-Typhimurium, non-4,[5],12:i:-	Salmonella, non-Typhimurium, non-4,[5],12:i:-
5	S. Typhimurium	S. Typhimurium
3	S. 4,[5],12:i:-*	Negative
2	S. 4,[5],12:i:-*	Inconclusive†
2	S. 4,[5],12:i:-	S. Typhimurium
1	Salmonella, non-Typhimurium, non-4,[5],12:i:-*	Negative

NVSL = National Veterinary Services Laboratories.

^*Few colonies to low levels of Salmonella sp. growth in culture.

^†Results would have been interpreted as “Inconclusive” given the high cycle threshold (Ct) values > 30.