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. 2019 Nov 21;10:1386. doi: 10.3389/fphar.2019.01386

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Copyright © 2019 Zhao, Chen, Hang, Li, Guo, Pan, Du, Zheng and Du

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein level of M3 muscarinic acetylcholine receptor (M3R) in transforming growth factor beta 1 (TGF-β1)-induced cardiac fibroblasts (CF). (A) Protein level of M3R in CF and cardiomyocytes (CM). n = 3. (B) Effect of TGF-β1 (20 ng/ml, 48 h) on messenger RNA level of TGF-β1. **p < 0.01 vs. Ctrl, n = 3. (C) Effect of TGF-β1 (20 ng/ml, 48 h) on mRNA level of collagen I. ***p < 0.001 vs. Ctrl, n = 3. (D) Effect of TGF-β1 (20 ng/ml, 48 h) on protein level of M₃R in CF. *p < 0.05 vs. Ctrl, n = 4.