Fig. 6.
Infection increases rolling, crawling, and adhesion of monocytes at the BBB. (A) Motility of individual tracked monocytes was classified based on speed and presented as a proportion of all monocytes. nPBS > 2,000 cells from 2 to 3 mice per time point, 4 to 5 FOV per mouse. nT. gondii > 2,000 cells from 3 to 5 mice per time point, 4 to 5 FOV per mouse. (B) Representative accumulation of CCR2-RFP cells with overlaid vessel traces (dotted lines) based on either tissue autofluorescence or intravenous injection of Alexa Fluor (AF) 488-Albumin. (Scale bars, 100 µm.) (C) Vessel accumulation was quantified as the area covered by CCR2-RFP fluorescence signal over the total area, inside and outside of the vessel boundaries (n = 3 to 4 mice per condition per time point with 3 FOV per mouse). *P < 0.05, ****P < 0.0001; significance was calculated by 2-way ANOVA with a Sidek post hoc test.