Fig. 3.

CircFGFR1 may function as a sponge for miR-381-3p. a RIP was performed for circRNA in A549 cells using a circFGFR1 probe and a negative control (NC) probe. b RIP experiments were carried out using an AGO2 antibody with NSCLC cell extracts. c Putative binding sites of miR-381-3p with respect to circFGFR1 were predicated via StarBase v3.0. d The luciferase activity of pLG3-circFGFR1 in the HEK-293 T cells after cotransfection with miR-381-3p. e The level of circFGFR1 in the streptavidin-captured fractions of the NSCLC cell lysates after transfection with biotinylated miR-381-3p or the negative control (NC). CircANRIL was used as a negative control. f miR-381-3p and circFGFR1 were detected by RNA in situ hybridization in the NSCLC cells. g and h The relative level of miR-383-3p was measured by RT-qPCR in the NSCLC cells transfected with circFGFR1, shcircFGFR1, or the control. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01