Table 1.

Primer sequences and PCR conditions.

Primer sequence	PCR conditions (n = 30 cycles)			Amplicon length	Restriction fragments
	D	H	E
Fwd: 5′CCGCAGCTCCCAACCACAATAAGCT3′	94°C for 30 s	56°C for 60 s	72°C for 60 s	253	TT 232; 21
					TC 253; 232; 21
Rev: 5′CAATAGGGCGAGGAGT3′
					CC 253

PCR: polymerase chain reaction; D: denaturation; H: hybridization; E: elongation; Fwd: forward primer; Rev: reverse primer.