Figure 6. . The differentiation into mature macrophages differentiated in the presence of the poly(acrylic acid)-coated iron oxide nanoparticles exhibits increased levels of IL-6 and IL-8 regardless of the exposure to the magnetic field.
MDMs were differentiated in the absence (open circles) or presence (filled circles) of PAC-IONs for 5 days and nonexposed or exposed to a 1.5-T MF (MF- and MF+, respectively) for 10 min. The levels of cytokines were evaluated by CBA in supernatants of cultures (A–F). The comparisons were made with a two-way ANOVA.
n = 5 independent experiments with three replicates each.
ANOVA: Analysis of variance; CBA: Cytometric bead array; MDM: Differentiation into mature macrophage; MF: Magnetic field; PAC-ION: Poly(acrylic acid)-coated iron oxide nanoparticle.