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. 2019 Oct 30;5(10):FSO423. doi: 10.2144/fsoa-2019-0066

Figure 7. . The differentiation into mature macrophages differentiated in the presence of the poly(acrylic acid)-coated iron oxide nanoparticles and exposed to the magnetic field shows a different pattern of cytokines when cocultured with autologous CD3+ T cells.

Figure 7. 

MDMs were differentiated in the absence (open circles) or presence (filled circles) of PAC-IONs for 5 days and nonexposed or exposed to a 1.5-T MF for 10 min. Then, MDMs were incubated for 6 days; supernatants were removed, autologous CFSE+ CD3+ T cells were added (1:2 ratio; MDMs:T cells) and stimulated with PHA or TT for 96 h. The levels of cytokines were evaluated by CBA in supernatants of co-cultures prepared with MDMs that had not been (A–F) or had been exposed to the MF (G–L). Comparisons were made with ANOVA II, p-values are shown in the figure; n = 5 independent experiments with three replicates each.

ANOVA: Analysis of variance; CBA: Cytometric bead array; MDM: Differentiation into mature macrophage; MF: Magnetic field; PAC-ION: Poly(acrylic acid)-coated iron oxide nanoparticle; PHA: Phytohemagglutinin; TT: Tetanus toxoid.