Table 2.

HTS protocol table

Step	Parameter	Value	Description
1	Controls	1 μl	DMSO (negative), buffer (positive) and untagged CAI (positive compound)
2	Library compounds	1 μl	50 μM, duplicate
3	Proteins	2 μl for each	Bio-CAI (30 nM), CA-CTD (30 nM)
4	Incubation time	30 min	Ambient temperature
5	Regents	2.5 μl for each	Anti-GST europium cryptate (3.63 nM) and XL665-conjungated streptavidin (0.75 nM)
6	Incubation time	1 h	Ambient temperature
7	Assay readout	665 nm and 620 nm	Envision multilabel reader, TR-FRET mode