Skip to main content
. Author manuscript; available in PMC: 2020 Jun 26.
Published in final edited form as: J Am Chem Soc. 2019 Jun 12;141(25):9964–9979. doi: 10.1021/jacs.9b03567

Figure 4.

Figure 4.

LC-MS chromatograms from the reactions of wild-type FtmOx1 (black) and its Y140F (purple), Y74F (blue), Y224F (green) and Y68F (red) variants. Reactions contained 0.010 mM protein, 0.010 mM Fe(NH4)2(SO4)2, 1.0 mM 2OG, 0.50 mM 2, and 1.0 mM ascorbate. The red arrow highlights the drastically diminished yield of verruculogen (3) uniquely in the reaction of the Y68F variant, and the asterisk marks the new major (as-yet-uncharacterized) product generated by this variant. The apparatus and conditions for the chromatographic analysis are provided in Experimental Procedures.