Skip to main content
. Author manuscript; available in PMC: 2020 Jun 26.
Published in final edited form as: J Am Chem Soc. 2019 Jun 12;141(25):9964–9979. doi: 10.1021/jacs.9b03567

Figure 8.

Figure 8.

Kinetic traces illustrating the complete suppression of both Tyr• absorption signatures by ascorbate. The panels show “dropline” treatments applied to the raw SF-abs data to extract the heights of the peaks (A) at 416 nm (reference wavelengths of 406 and 426 nm), arising from the first Tyr• and (B) at 410 nm (reference wavelengths of 404 and 416 nm), arising from the second Tyr•. The reactions were carried out by mixing an anoxic solution of 0.65 mM protein, 0.58 mM Fe(NH4)2(SO4)2, 0.58 mM 2, 5.0 mM 2OG, and (solely for the blue traces) 1.0 mM ascorbate at 5°C with an equal volume of O2-saturated buffer.