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. 2019 Dec 5;12:10671–10679. doi: 10.2147/OTT.S226040

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© 2019 Wang et al.

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The expression of lncRNA HULC is higher in breast cancer tissues and cell lines. (A) Relative expression of HULC in breast cancer cell lines and normal breast cell line was examined by qRT-PCR. Breast cancer cell lines: MCF-7, BT-20, ZR-75-1, MDA-MB-231. Normal breast cell line: MCF-10A. Data were normalized to 18S and expressed as mean ± SD. (B) Relative expression of HULC in breast cancer tissues relative to adjacent normal breast tissues was examined by qRT-PCR. n=60. Data were normalized to 18S and expressed as mean ± SD. (C) Relative expression level of HULC was examined using qRT-PCR in breast cancer tissues with non-metastasis (n=35) and metastasis (n=25). Data were normalized to 18S and expressed as mean ± SD. (D) Relative expression level of HULC was examined using qRT-PCR in breast cancer tissues with stage I-II (n=28) or stage III-IV (n=37). Data were normalized to 18S and expressed as mean ± SD. (E) Overall survival rate of HULC was analyzed by Kaplan–Meier analysis coupled with log-rank test in breast cancer. Higher HULC expression level group (n=33). Lower HULC expression level group (n=27). (P=0.032). *P<0.05.