Figure 7.

Effect of vit. D-NPs and Ab-vit. D-NPs on serum calcium, phosphate, and aortic phosphate content. C57BL/6 mice received intraperitoneal (i.p.) injection daily of 1α, 25(OH)₂D₃, vit. D-NPs, and Ab-vit. D-NPs (0.002 μg/g BW of vitamin D3) (n=3 in each group). Mice received daily i.p. instillation of phosphate-buffered saline (PBS) in the control group. Blood samples and aorta tissue samples were taken after 21 days. (A) In the Ab-vit. D-NPs group, the hypercalcemia side effect was significantly lower than the vitamin D group. (B) No significant difference was found in the serum phosphate of the different groups. (C) The aortic phosphorus content showed a trend, though no significant difference of Ab-vit. D-NPs alleviating aortic phosphate deposition. (Data are represented as mean ± SD, n=3; **P<0.01, ***P<0.001, compared with the control group; ^###P<0.001, compared with the vitamin D group).