Table 1:

N-linked glycans identified in the re-analysis

m/z	Centers	t-Statistics	Adjusted P-values	M+Na+	Composition	ppm
1,257.47424	38.24	51.97	0	1,257.41	(Hex)2+(Man)3(GlcNAc)2	51
1,743.68713	32.11	48.56	0	1,743.57	(Hex)5+(Man)3(GlcNAc)2	67
1,419.55334	40.68	48.2	0	1,419.47	(Hex)3+(Man)3(GlcNAc)2	59
1,905.68713	48.61	44.78	0	1,905.63	(Hex)6+(Man)3(GlcNAc)2	30
2,304.91211	43.53	42.36	0	2,304.83	(Hex)2(HexNAc)3(deoxyhexose)3+(Man)3(GlcNAc)2	36
1,850.71216	25.3	42.01	0	1,850.65	(Hex)1(HexNAc)3(deoxyhexose)1+(Man)3(GlcNAc)2	34
1,581.62573	18.07	40.64	0	1,581.53	(Hex)4+(Man)3(GlcNAc)2	61
1,809.72461	10.81	38.15	0	1,809.63	(Hex)2(HexNAc)2(deoxyhexose)1+(Man)3(GlcNAc)2	52
2,158.88721	14.77	38.03	0	2,158.77	(Hex)2(HexNAc)3(deoxyhexose)2+(Man)3(GlcNAc)2	54
1,663.66638	10.27	32.26	0	1,663.57	(Hex)2(HexNAc)2+(Man)3(GlcNAc)2	58
1,688.71509	8.68	28.29	0	1,688.61	(HexNAc)3(deoxyhexose)1+(Man)3(GlcNAc)2	62
1,485.62378	8.67	26.89	0	1,485.53	(HexNAc)2(deoxyhexose)1+(Man)3(GlcNAc)2	63
2,012.78394	7.3	26.72	0	2,012.71	(Hex)2(HexNAc)3(deoxyhexose)1+(Man)3(GlcNAc)2	37
2,816.11206	6.92	26.35	0	2,816.01	(Hex)3(HexNAc)4(deoxyhexose)1+(Man)3(GlcNAc)2	36
2,067.75903	5.69	14.52	0	2,067.67	(Hex)7+(Man)3(GlcNAc)2	43
1,542.61902	5.59	8.08	0	1,542.55	(HexNAc)3+(Man)3(GlcNAc)2	45

We could identify 16 N-linked glycans by matching the m/z features of the MSI data (col. 1) to the identified m/z features of the LC-MS/MS experiment (col. 5). We allowed a maximum tolerance of 300 ppm and multiple matches. Only single matches occurred with an average m/z error of 46 ppm (col. 6). Centers, t-statistics and adjusted p-values obtained by the spatial shrunken centroid algorithm are reported in column 2-4. Glycan composition in column 6: Hex: Hexose, Man: Mannose, GlcNAc: N-Acetyl-D-glucosamine, HexNAc: N-Acetyl-D-hexosamine