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αEnNaC−/− mice prevents WD-induced an increase of inflammation and reduction of AMPKα and eNOS activity and Sirt1 expression. (A) mRNA levels of ICAM-1, VCAM-1, IL6, MCP-1. (B) The protein abundance of AMPKα, eNOS, Sirt1 and GAPDH in aortic tissues were performed with immunoblotting. Quantitative analysis of protein abundance in p-AMPKα (C), p-eNOS (D), and Sirt1 (E). † p<0.05 compared with CD αEnNaC+/+ group; ‡ p<0.05 compared with WD αEnNaC+/+ group in multiple comparison analysis.
