Table 1. Primer and probe sequences used in this study.
Serotype | Forward Primer | Reverse Primer | Probe |
---|---|---|---|
DENV-1 | ACC GTC TTT CAA TAT GCT GAA ACG | GGT ATG GCT AGA AAT CTT AGG AAT G | 6FAM–TTC ACA GTT GGC GAA GAG ATT CTC AAA AGG AT–TAMRA |
DENV-2 | GCT GAA ACG CGA GAG AAA CC | CAG TTT TAT TGG TCC CG TCC CT | 6FAM–AGC ATT CCA AGT GAG AAT CTC TTT GTC AGC TGT—TAMRA |
DENV-3 | GCT GAA ACG CGT GAG AAA CC | CAA TTT CAT TGG TCC CTG GCC GT | 6FAM–AGC AGT CCT TTT GAG AAT CTC TTC–TAMRA |
All sequences are listed 5’ to 3’. DENV-2 primer and probe sequences were obtained from [31]. All other primers and probes were designed using similar to logic that used in deriving primers for DENV-2.