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The shape of released protoplasts of Colletotrichum scovillei KC05. The average size of protoplasts was 8.82 ± 0.06 μm after 3 h incubation and 11.09 ± 0.07 μm after 5 h incubation in the 2% lysing enzyme and 0.8% driselase mixture. Lysed protoplasts were observed after 5 h incubation under a differential interference contrast microscope. Scale bar = 50 μm.
