Table 2.
Summary of transformation efficiencies and generation of knock-out mutants for the 19 loci in Colletotrichum scovillei strain KC05
| Target locus | Size of target locus for homology-dependent replacement (bp) | No. of transformants tested for PCR screening | No. of positive candidates for knock-out mutants in PCR screening | Percentage of positive candidates in PCR screening | No. of knock-out mutants confirmed in Southern blots |
|---|---|---|---|---|---|
| CSP_000836 | 2,413 | 95 | 15 | 15.7 | 2/3 |
| CSP_001376 | 2,685 | 114 | 15 | 13.1 | 2/3 |
| CSP_000583 | 2,262 | 118 | 18 | 15.2 | 2/2 |
| CSP_001741 | 2,727 | 103 | 12 | 11.6 | 2/3 |
| CSP_002212 | 638 | 138 | 20 | 14.4 | 3/3 |
| CSP_004175 | 1,982 | 109 | 19 | 17.4 | 3/3 |
| CSP_003850 | 2,295 | 121 | 21 | 17.3 | 3/3 |
| CSP_003491 | 585 | 119 | 26 | 21.8 | 1/5 |
| CSP_005482 | 1,407 | 88 | 17 | 19.3 | 2/3 |
| CSP_005764 | 3,789 | 113 | 17 | 15.0 | 2/2 |
| CSP_006569 | 1,883 | 111 | 8 | 7.2 | 3/3 |
| CSP_006607 | 556 | 106 | 25 | 23.5 | 3/5 |
| CSP_007586 | 678 | 45 | 5 | 11.1 | 5/5 |
| CSP_008738 | 970 | 81 | 12 | 14.8 | 2/4 |
| CSP_009802 | 496 | 111 | 18 | 16.2 | 2/5 |
| CSP_010617 | 3,976 | 93 | 26 | 27.9 | 3/3 |
| CSP_009947 | 303 | 108 | 18 | 16.6 | 1/2 |
| CSP_010985 | 2,028 | 81 | 24 | 29.6 | 3/3 |
| CSP_011033 | 1,260 | 113 | 21 | 18.5 | 2/2 |