Fig. 5.

Validation of the loop-mediated isothermal amplification (LAMP) assay using field samples. (A) Peach leaves and fruit samples collected from a peach orchard. Red arrows indicate the diseased areas. No. 1–10 indicate the following varieties of peach: Kanoiwa Hakuto, Changbangjosaeng, Baekmijosaeng, Cheonhong, DaehwaJosaeng, Daemyeong, Baekmijosaeng, Hakuho, Sulwooro 4–55, and Hikawa Hakuho, respectively; No. 11–13: peach fruit. (B) Electrophoresis analysis and visual analysis using a UV illuminator. M: 1 kb plus DNA ladder; P: positive control; N: negative control; Lanes 1–13 represent the LAMP results for samples No. 1–13, respectively. (C) Electrophoresis analysis of the LAMP products digested by HaeIII. P: positive control; Lanes 1–7 indicate the LAMP products digested by HaeIII from Daemyeong, Baekmijosaeng, Sulwooro 4–55, Hikawa Hakuho, and peach fruit 11–13, respectively.