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mRNA expression of ADM and its receptor components by VAT. VAT was obtained from HFHS or CD fed mice treated with or without ADM22–52. qPCR was performed to determine the mRNA expression of (A) Adm, (B) Crlr, (C) Ramp2, and (D) Ramp3. mRNA was normalized to the average of three housekeeping genes, β-actin, Gapdh and Hprt and data displayed as mean+/−SEM. Different letters indicate significant difference between groups (p<0.05).
