Effect of HFHS and ADM22–52 on the expression of lipolytic enzymes. VAT was obtained from mice under HFHS or CD diets treated with or without ADM22–52. qPCR was performed to determine the mRNA expression of (A) Plin1, (B) Hsl, and (C) Lpl,mRNA was normalized to the average of three housekeeping genes, β-actin, Gapdh and Hprt and data displayed as mean+/−SEM. Different letters indicate significant difference (p<0.05).