Figure 5.

RAN translation inhibitors have varying effects on AUG-initiated repeat translation. A, schematic of NLuc reporter mRNAs used in 30% RRL in vitro translation assays, illustrating the position of the AUG start codons inserted upstream of the repeats, to drive translation in the indicated reading frames. The effect of anthralin (B), TMPyP4 (C), protoporphyrin IX (D), BIX01294 (E), and CP-31398 (F) on +1 and +2CGGx100 RAN translation, compared with AUG-initiated CGGx100 translation, measured in the presence of increasing concentrations of each small molecule. For B–F, values are relative to DMSO controls, and graphs represent the mean of n = 3. Error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test. 3xF, 3xFLAG tag.