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. 2019 Oct 30;294(49):18742–18755. doi: 10.1074/jbc.RA119.010973

Figure 1.

Figure 1.

ICC to show the interaction of ZIKV with centrosome proteins. A1–A4, relationship of PCNT and PCM1 in SK-N-SH cells. A1, merged image; A2, PCNT to show the centriole; A3, PCM1 to show the PCM1 granules; A4, DAPI to show the nuclei. B, effects of ZIKV MR766 on PCM1 granules in SK-N-SH cells. B1, merged image; B2, E protein to show the viral infection; B3, PCM1 to show the PCM1 granules; B4, DAPI to show the nuclei. C, effects of ZIKV MR766 on centriole in SK-N-SH cells. C1, merged image; C2, E protein to show the ZIKV infection; C3, PCNT to show centriole; C4, DAPI. D, distribution of PCM1 and PCNT in different cells. ICC was performed to show the relationship of PCNT (green) and PCM1 (red) in the cell line as indicated. PCNT and PCM1 colocalize in all of the cells: U251 MG, MRC-5, Vero, ARPE-19, and NSC. E, percentile of the cells in which the PCM1 was dispersed in different cells: MRC-5, U-251 MG, SK-N-SH, Vero, ARPE-19, and NSC. The degree of significance was as follows: *, p < 0.001. F, same as B, but photographs were taken with a ×40 lens so that more cells could be visualized. Scale bar, 10 μm. Error bars, S.E.