Figure 11.

ZIKV infection resulted in reduction of pericentrosomal proteins: PCM1, CEP290, USP9X, and CEP131. Western blotting assay to examine the levels of PCM1 and Mib1 in SK-N-SH cells that were infected or mock-infected with ZIKV at an MOI of 1 for 24, 48, or 72 h. NS3 was to show viral infection, and tubulin was used to control for sample loading. Left, without MG132 treatment; right, with MG132 treatment 12 h before collecting samples. We have performed two independent experiments; the densitometry was obtained by averaging the two Western blot analyses, and the numbers represent the mean protein expression. The densities of protein bands are first normalized with tubulin, and then the proteins from ZIKV-infected groups were compared with these from mock-infected groups. If the ratio is <1, it means that the protein is reduced by ZIKV.