Figure 12.

Effects of ZIKV on PCM1 in Mib1 knockout cells. SK-N-SH cells were transfected with a mixture of CRISPR/Cas9 plasmids that target the Mib1 gene, and the GFP-positive cells were selected and diluted to generate Mib1-ko cells. A, left, Western blot analysis to test the protein level of Mib1 in SK-N-SH and its Mib1-ko cells; right, ICC to test Mib1 and PCM1 production in SK-N-SH and Mib1-ko cells. B, left, ZIKV (MR766 or PRVABC59) was infected into Mib1-ko or normal SK-N-SH cells for 24 h at an MOI of 1. The whole-cell lysates were collected to run a Western blotting assay to detect PCM1, Mib1, NS3, and tubulin. Right, co-transfections of plasmids expressing protein as indicated into Mib1-ko cells for 24 h. Western blots were performed to examine each protein as indicated on the right. C, ICC to show whether ZIKV infection disperses PCM1 in either SK-N-SH or Mib1-ko cells.