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ICC to examine the interaction of ZIKV PRVABC59 and cellular proteins. SK-N-SH cells were grown on coverslips and infected with ZIKV PRVABC59 at an MOI of 0.5 for 24 h. The cells were then fixed for IFA to check viral protein (NS3) and cellular proteins. A, CEP135, to show centriole; B, α-tubulin; C, acetyl-α-tubulin; D, β-actin. Top, ZIKV-infected cells; bottom, mock-infected cells.
