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MIF-2 candidate inhibitors and 4-CPPC selectivity for MIF-2. A, chemical structures of the top seven hits identified by virtual screening of candidate MIF-2 inhibitors. B, the HPP tautomerization assay was performed with increasing concentrations of 4-CPPC for both MIF-1 (left panel) and MIF-2 (right panel), showing 17-fold selectivity at the point of 50% inhibition.
