Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 2;294(49):18522–18531. doi: 10.1074/jbc.RA119.009860

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Tilstam et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 6. — Functional inhibition of MIF-1 and MIF-2 by MIF098 and 4-CPPC. A, MIF098 selectively inhibits MIF-1 tautomerase activity compared with MIF-2 measured by HPP tautomerization. B and C, MIF-2–CD74 (B) and MIF-1–CD74 (C) binding assay using biotinylated human MIF-2 or biotinylated human MIF-1 and immobilized recombinant sCD74. Anti-MIF-1 antibody, anti-MIF-2 antibody, human MIF-1 (hMIF-1), human MIF-2 (hMIF-2), 4-CPPC, and MIF098 were added at increasing concentrations, and biotinylated MIF-2 binding/MIF-1 binding to sCD74 was revealed by detection of streptavidin-conjugated alkaline phosphatase. Nonreactive IgG served as a negative control. D and E, selective inhibition by 4-CPPC or MIF098 of MIF-2 (D) and MIF-1 (E) stimulated phosphorylation of ERK1/2 in cultured human fibroblasts.