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ERAP1-mediated trimming of free 14-mer (RA)3AAKKKYCL and 20-mer (RA)6AAKKKYCL. Free peptides (∼12 μg) were incubated with 0.4 μg of ERAP1 at 37 °C. An aliquot was taken after 20 min (14-mer) and 30 min (20-mer) and analyzed by MS. The starting peptides and their fragments are indicated.
