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Hemithioacetals can be decomposed by DJ-1, Glx3, and Tris through the removal of free MGO. 5 mm MGO was mixed with 5 mm NAC and incubated for 10 min before the addition of DJ-1 (A), the C106S mutant of DJ-1 or Tris (B), or Glx3 (C) at the indicated final concentrations. Protein absorbance at 288 nm was subtracted from all samples.
