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In vivo reconstitution of AtPRFs in budding yeast. A, constructs of yeast expression cassette for AtPRF isoforms. The lengths of UTRs were indicated. B, tetrad analysis of heterozygous diploid ScPFY1/AtPRF. Haploids expressing AtPRF1 and AtPRF3 were highlighted by squares. C, growth of haploid strains expressing ScPFY1 and AtPRFs on YPD plate at 25 °C for 48 h.
