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. 2019 Oct 25;294(49):18650–18661. doi: 10.1074/jbc.RA119.011307

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© 2019 Qiao et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Functional validation of AtPRF3Δ22 variants. A, kinetic actin polymerization of AtPRF3Δ22, AtPRF3Δ22M1, and AtPRF3Δ22M2 in the presence of AtFH1-FH1FH2COOH (AtFH1). B, thermostatted microplate reader measures the growth of yeast strains expressing AtPRF3Δ22 mutants and WT. C, actin cables motility in heterozygous diploid strains ScPFY1/AtPRF3Δ22, ScPFY1/AtPRF3Δ22M1, and ScPFY1/AtPRF3Δ22M2 (n = 100 filaments). p values were determined by two-tailed Student's t test assuming equal variances (*, p < 0.05; ****, p < 0.0001). Error bars represent S.D. D, lifetimes of Abp1-mRFP patches in the indicated haploids. n = 100 patches used for analysis.