Figure 2.

Establishment and erasure of specific H3K9me3 modifications during mouse spermatogenesis. A, heat map showing the dynamics of H3K9me3 domains during mouse spermatogenesis. The colors represent the log 2–transformed H3K9me3/input ratios scaled by row. For each cluster, the average distances to the TSS and LTR regions are also plotted. The colors represent distance (log₁₀). B, box plots of the H3K9me3 signals of the mitosis-specific, PS- and RS-specific, and sperm-specific domains of H3K9me3-covered genes. The middle lines in the boxes represent the medians, and the lower and upper lines represent the 5 and 95% quantiles, respectively. C, box plots of the gene expression levels of the mitosis-specific, PS- and RS-specific, and sperm-specific domains of H3K9me3-covered genes. The middle lines in the boxes represent the medians, and the lower and upper lines represent the 5 and 95% quantiles, respectively. D, box plots of the DNA methylation levels of the mitosis-specific, PS- and RS-specific, and sperm-specific domains of H3K9me3-covered genes during mouse spermatogenesis. E, integrative genomics viewer snapshot of H3K9me3 signals and the absolute DNA methylation levels of the Mgst3 gene. The signals are presented as the log 2–transformed H3K9me3/input ratios.