Figure 2.

Ca²⁺-dependent recognition of dead cells by SCARA1. A, mSCARA1 binds to the ActD-treated NIH 3T3 cells in the presence of Ca²⁺. Mock represents untreated viable cells. B, hSCARA1 binds to the ActD-treated Jurkat cells in the presence of Ca²⁺. C, ELISA data show that mSCARA1 binds to the Jurkat cell lysates in the presence of Ca²⁺. D, confocal image of the permeabilized NIH 3T3 cells stained by GFP–mSCARA1 and DAPI with Ca²⁺ (scale bar, 25 μm). E, confocal image of the permeabilized NIH 3T3 cells stained by GFP–mSCARA1 and DAPI with EDTA (scale bar, 25 μm). F, staining of viable (left) and the ActD-treated (middle) Jurkat cells by annexin V–APC, PI, and GFP–mSCARA1 in the presence of Ca²⁺. The binding of GFP–mSCARA1 to the gated subsets of viable and the ActD-treated Jurkat cells are shown on the right. Mock represents untreated viable cells.