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. 2019 Oct 29;294(49):18898–18908. doi: 10.1074/jbc.RA119.011248

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© 2019 Sasaki et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Effects of YidC mutants on MPIase-dependent integration. A, effects of YidC mutants on 3L-Pf3 coat integration. 3L-Pf3 coat was in vitro synthesized in the presence of (proteo)liposomes containing MPIase and the specified YidC variant. Cold methionine (0.3 mm) was added to synthesize 3L-Pf3 coat at high level. The integration activities are indicated at the bottom. B, effects of YidC mutants on Pf3 coat integration. Pf3 coat was in vitro synthesized in the presence of (proteo)liposomes as described in (A). The integration activities are indicated at the bottom. C, effects of Bh-YidC and its mutant on Pf3 coat integration. Pf3 coat was in vitro synthesized in the presence of (proteo)liposomes containing MPIase and/or Bh-YidC or the R72Q mutant, as specified. The integration activities are indicated at the bottom. All the integration activities with S.D. were calculated for three independent experiments.