Table 1.
ITC-binding constants and thermodynamic parameters for MA protein interactions with NDs and Fab
Titrations were conducted in a buffer containing 50 mm sodium phosphate (pH 6.0) and 50 mm NaCl.
| Titrations | n | Kd | ΔH° | −TΔS° | ΔG° |
|---|---|---|---|---|---|
| μm | kcal/mol | kcal/mol | kcal/mol | ||
| MA into POPC:POPS:PI(4,5)P2 (75:20:5) NDs | 10.3 ± 0.2 | 10.3 ± 1.9 | −4.3 ± 0.3 | −2.5 ± 0.4 | −6.8 ± 0.1 |
| MA122FD into Fab | 1.00 ± 0.03 | 0.060 ± 0.005 | −3.2 ± 0.2 | −6.8 ± 0.2 | −10.0 ± 0.1 |
| MA122FD-Fab into POPC:POPS:PI(4,5)P2 (75:20:5) NDs | 6.0 ± 0.2 | 0.32 ± 0.03 | −5.7 ± 1.1 | −3.3 ± 1.1 | −9.0 ± 0.1 |