Figure 3.

The N-terminal region of Rif2 is important for its inhibitory role. A, standard kinase reaction with increasing concentrations of either Rif2-WT or Rif2-F8A. A representative experiment is shown. B, quantification of three independent experiments with S.E. Relative Tel1 activity of the reaction without Rif2 was set to 100 (lane 2). C, standard kinase assays contained 2 μm indicated Rif2 or GST-Rif2 variants. D, standard kinase assays were carried out without DNA and Rif2 as indicated. Reactions were incubated for 30 min. Bottom, averages and S.E. are derived from three independent experiments. The reaction without Rif2 (lane 1) was set to 100.