Skip to main content
. 2019 Oct 21;294(49):18586–18599. doi: 10.1074/jbc.RA119.010950

Figure 2.

Figure 2.

Identification of estrogen-deconjugating β-glucuronidase enzymes. A, sequence similarity network of 279 unique GUS enzymes from the Human Microbiome Project. Each circle represents a unique protein sequence. Of the 279 total proteins shown, 35 enzymes were tested for their ability to reactivate estrone-3-glucuronide and estradiol-17-glucuronide (triangles). Colored triangles represent those enzymes that can reactivate estrogens, gray triangles show those that cannot. Colored squares, the two novel GUS proteins identified here that can reactivate estrogen glucuronides (PDB: 6U7J and 6U7I). B, catalytic efficiencies of 17 GUS enzymes that reactivate estrone-3-glucuronide. Red bars, Loop 1 enzymes; green bars, mini-Loop 1 enzymes; yellow bars, FMN-binding GUS enzymes. F. prausnitzii (Loop 1) through F. prausnitzii L2–6 (FMN) show statistically significant difference at a p value of 0.001 (*) compared with E. eligens and S. agalactiae. Clostridium sp. Marseille-P299 (Loop 1) through F. prausnitzii (mini-Loop 1) show statistically significant difference at a p value of 0.0001 (**) compared with E. eligens (Loop 1) and S. agalactiae (Loop 1). C, catalytic efficiencies of 15 GUS enzymes that reactivate estradiol-17-glucuronide. Red bars, Loop 1 enzymes; green bars, mini-Loop 1 enzymes; yellow bars, FMN-binding GUS enzymes. R. gnavus 3 (FMN) through F. prausnitzii L2–6 (FMN) show statistically significant difference at a p value of 0.001 (*) compared with S. agalactiae (Loop 1) and C. perfringens (Loop 1). Uncultured Clostridium sp. (Loop 1) through F. prausnitzii (mini-Loop 1) show statistically significant difference at a p value of 0.0001 (**) in comparison to S. agalactiae (Loop 1) and C. perfringens (Loop 1). Data are presented as the average of 3 biological replicates ± SEM.