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. 2019 Dec 3;7:367. doi: 10.3389/fbioe.2019.00367

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Copyright © 2019 Pan, Sun, Tang, Liu, Zhang, You, Osire, Sun, Zhao, Xu, Yang and Rao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of DacA on prodigiosin-synthesis gene pigA expression and cell shape. (A) Gene organization of the prodigiosin biosynthetic gene clusters in S. marcescens. These 14 genes were transcribed as a polycistronic mRNA from a promoter upstream of pigA gene. (B) RT-qPCR analysis of the pigA gene expression level in the strains JNB5-1 and dacA mutant SK4-72. (C) SEM images of the cells of JNB5-1, SK4-72, and SK4-72/pXW1803 strains. (D) Measurements of cell length and width of the indicated cells (40 cells for each strains). For (B), the experiment was performed in biological triplicates. Error bars indicate the standard deviations. For (D), one-way ANOVA was used to examine the mean differences between the data groups. ****p < 0.0001; ns, No significant difference.