Table 2.

Primers used in this study.

Primer	Primer sequence (5′-3′)	Function
OTn1	GATCCTGGAAAACGGGAAAG	Identification of Tn5G in prodigiosin production mutants
OTn2	CCATCTCATCAGAGGGTAGT
RT-pigA-F	ATGGCTTTATGGGCGTGTCC	qPCR primers, coding region of pigA gene
RT-pigA-R	GAGAGGCAATCTTTCGGGCT
RT-16S-F	CACACCGCCCGTCACACCA	qPCR primers, coding region of 16S rRNA gene
RT-16S-R	CGCAGGTTCCCCTACGGTTAC
DacA-F	TTAACCGAACCAGTGGTGGAACATC	Amplification of dacA gene with its native promoter
DacA-R	TTAAGCCCGGCGGGCATTCA
DacB-U-F	TAGGCCGAATTCGAGCTCGGTACCCGG ACAGGGTTTCGTCCACC	Amplification of dacB gene upstream homologous arms
DacB-U-R
DacB-D-F		Amplification of dacB gene downstream homologous arms
DacB-D-R	ATTACAGCCGGATCCCCGGGTACCG TAAAAATCGCCGCTGAAGATCGC
DacC-U-F	TAGGCCGAATTCGAGCTCGGTACCGAT GGCGTCCACGTCATGC	Amplification of dacC gene upstream homologous arms
DacC-U-R
DacC-D-F		Amplification of dacC gene downstream homologous arms
DacC-D-R	ATTACAGCCGGATCCCCGGGTACCTCGACTTTGGGCAGATCCGC
DacD-U-F	TAGGCCGAATTCGAGCTCGGTACCGTGTCGCCCACCAGCTTAGG	Amplification of dacD gene upstream homologous arms
DacD-U-R
DacD-D-F		Amplification of dacD gene downstream homologous arms
DacD-D-R	ATTACAGCCGGATCCCCGGGTACCGGC GGCGTTTTTCAGGCTG
Apr-D-F	CGCGGAACCCCTATTTGTTTATTTTTC	Amplification of aacC3 gene
Apr-D-R	TCAGCCAATCGACTGGCGAGC
DacB-F	CCTGCTTTTCCATCAGTTCCAGG	Primers used to confirm dacB gene knockout succeed
DacB-R	ATCAGTCCAAGTGGCCCATCTTC
DacC-F	GCCGATGCGGTAAGCATTGC	Primers used to confirm dacC gene knockout succeed
DacC-R	ATCAGTCCAAGTGGCCCATCTTC
DacD-F	GGCCTTTATTATCGGCCAAACGG	Primers used to confirm dacD gene knockout succeed
DacD-R	ATCAGTCCAAGTGGCCCATCTTC

The sequences underlined stand for the homologous sequence of the pUTKm plasmid.

The sequences wavelined stand for the homologous sequence of the apramycin resistance aacC3 gene.