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. 2019 Dec 3;10:1455. doi: 10.3389/fphys.2019.01455

Figure 4.

Figure 4

Disruption of actin dynamics insulted tight junction (TJ) proteins. (A,B) Caco-2 cells were pretreated with jasplakinolide or latrunculin A (LatA) for 0, 0.25, 0.5, 1, 2, 6, and 24 h, respectively. Cell lysates were analyzed to detect the expressions of zonula occludens (ZO)-1 and occludin. The immunoblot assay showed the relative protein levels of the ZO-1 and occludin after pretreatment with jasplakinolide (A) or LatA (B). ap < 0.05 compared with 0 h group. (C,D) Caco-2 cells were pretreated with jasplakinolide or LatA for 24 h, respectively. The proteins were labeled with Texas red. The cellular distribution of ZO-1 (C) and occludin (D) along the cell envelope decreased, with morphological changes, such as folds, serrations, and breaks. Scale bar = 10 μm. Data are representative of five similar experiments.