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. 2019 Dec 9;5:150. doi: 10.1038/s41420-019-0234-y

Fig. 4. Cellular metabolic events.

Fig. 4

a Cells were charged with calcein-AM and either FAC (200 μM) or Fe-NTA (100 μM) and compared with cells loaded with curcumin only (20 μM). Cells were simultaneously treated with FAC and curcumin or Fe-NTA and curcumin. After 1 h, the cells were rinsed with phosphate buffer saline (PBS) on ice and calcein fluorescence was measured. (±SD for six repeated experiments). b Effect of iron loading by cell loading with 0–500 μM Fe-NTA and its effect on glutathione activity.(±SD for eight repeated experiments). c Effect of iron loading (as in d) on lipid peroxidation as a function of time (0–120 h). d Effect of iron loading (as in d) on protein carbonylation as a function of time (0–120 h). e Measurement of high doses of iron loaded with 250 µM Fe-NTA in the presence of 20 µM curcumin. f Measurement of high doses of iron loaded with 500 µM Fe-NTA and 20 µM or 50 µM curcumin. All experiments presented in c to f are the mean ± SD for 10 repeated experiments.