Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 9;5:150. doi: 10.1038/s41420-019-0234-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Flow cytometry analysis of Huh-7 cell death. Percentage of viable, apoptotic and necrotic cells among cells treated with various concentrations of curcumin (0–50 μM for 24-h incubation). YO-PRO-1/PI staining was used to analyze membrane permeability and discriminate three populations: YO-PRO-1⁻/PI⁻ for viable cells, YO-PRO-1⁺/PI⁻ or YO-PRO-1⁺/PI intermediate for apoptotic cells and YO-PRO-1⁺/PI⁺ for necrotic cells. Asterisk inidicates the maximal number of apoptotic cells was observed at 25 μM curcumin for 24 h. Data are expressed as the mean ± S.D. (n = 7). b Same as in panel a but plotted as a function of time from 0 to 24 h for 25 µM curcumin. Data are expressed as the mean ± S.D. (n = 7). c Metabolic measurements linked to dying cells after 24-h incubation with 25 µM curcumin. Mitochondrial membrane potential with DiOC₆(3) fluorescence (ΔΨm, black circles), superoxide anion generation tested with MitoSOX-green fluorescence (O₂^.−, empty circles) and calcium increase measured with Fluo 4-AM (intracellular calcium, red diamonds). Data are expressed as the mean ± S.D. (n = 10).