Table 1.
Regulation of the main metabolic events linked to ferroptosis induction.
| Treatment | Control cells | + Fe-NTA 500 µM | + Fe-NTA 500 µM +Curcumin 20 µM | + Fe-NTA 500 µM +Curcumin 50 µM |
|---|---|---|---|---|
| Iron amount µg/g cells | 7.2 ± 1,9 | 114 ± 10.6 | 109 ± 9.5 | 109 ± 19.5 |
| Gluthatione activity (a.u.) | 74 ± 8,2 | 17 ± 5.3 | 39 ± 8.4 | 69 ± 7.9 |
| GPX4 activity a.u./mg protein | 370 ± 25.2 | 28 ± 7.2 | 130 ± 31.9 | 350 ± 29.4 |
| Lipid peroxidation a.u. X 100 | 1.6 ± 0.3 | 7,3 ± 1.4 | 3,9 ± 0,9 | 1,7 ± 0.2 |
| Protein carbonylation nmole/mg protein | 17.3 ± 0.5 | 67,5 ± 8,7 | 29,9 ± 7.2 | 17,1 ± 0.4 |
| Necroptosis (% of cells PI +++) | 9 ± 3.2 Necrosis in culture | 90 ± 6.4 | 39 ± 7.3 | 14 ± 5.9 |