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. 2019 Dec 9;10(12):940. doi: 10.1038/s41419-019-2134-8

Fig. 4. Intra-mitochondrial Src has specific effects on viability and cell cycle in MDA-MB-231 cells.

Fig. 4

a Flow cytometry analyses (left) and quantification (right) of MDA-MB-231 cells expressing empty vector (pcDNA), Src-HA or MLS-Src-HA, treated with vehicle or Actinomycin D (5 µM, 48 h) and stained with PI and Annexin V-FITC (n = 6), showing that overexpression of mtSrc in MDA-MB-231 decreases the number of viable cells and block early apoptosis upon treatment with Act. D. b Flow cytometry analyses (left) and quantification (right) of MDA-MB-231 cells expressing empty vector (pcDNA), Src-HA or MLS-Src-HA labeled with Ki67 and PI (n = 7–13), indicating that overexpression of mtSrc decreases the number of cells in G1 phase. Data are presented as mean ± s.e.m. For A, ***p ≤ 0.001 as compared to pcDNA; ##p ≤ 0.01, ###p ≤ 0.001 as compared to vehicle (veh), according to two-way ANOVA followed by Dunnett’s multiple comparison test. For b, **p ≤ 0.01 as compared to pcDNA according to two-way ANOVA followed by Dunnett’s multiple comparison test.