Fig. 2.

SIRT2 inhibition upregulates GluN2A, GluN2B, and GluA1 expression in 7-month-old SAMR1 and SAMP8 mice. No differences were found concerning hippocampal protein levels of Beclin 1 (a) and ROCK2 (b) (n = 7). c SAMP8 show higher levels of LC3-II (n = 7). d Representative image and quantitative measurement of hippocampal ubiquitinated proteins (n = 7) (F = 6.454, p < 0.05, main effect of strain, two-way ANOVA). Effect of 33i on hippocampal GluN2A (e), GluN2B (f), and GluA1 (g) expression (n = 6–8). *p < 0.05 (F = 8.724, p < 0.05; F = 6.487, p < 0.05; F = 30.40, p < 0.05, respectively, main effect of treatment, two-way ANOVA). Results show that 33i treatment increases the gene expression of h Glun2a (F = 6.436, p < 0.05, main effect of treatment) and i Glun2b (F = 6.496, p < 0.05, main effect of treatment) but not of Glua1 j (n = 5–8). Results are shown as mean ± SEM