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. 2019 Dec 9;9:18575. doi: 10.1038/s41598-019-54960-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification and quantification of CTCs isolated from HCC patients. (A) Representative image of CTCs captured by the Labyrinth and stained using antibodies against Gly3, GS, Hep Par-1, CD45, and DAPI. Scale bar represents 10 μm. (B) Number of CTCs/mL in HCC patients grouped by TNM stage. **TNM stage 0-I vs non-HCC: p = 0.0019; ***stage II-IV vs non-HCC: p = 0.0001; stage 0-I vs II-IV, p = 0.1258 (ns). (C) CTC positivity rate in TNM stage. **non-HCC vs Stage 0-I: p = 0.0031; ***non-HCC vs Stage II-IV: p < 0.0001; *Stage 0-I vs II-IV: p = 0.0398. (D) CTC positivity rate in BCLC stage. **non-HCC vs Stage 0-A: p = 0.0048; ***non-HCC vs Stage B-C: p < 0.0001; Stage 0-A vs Stage B-C: p = 0.3126 (ns).